9+ tet-plko-puro vector

ZERO BIAS - scores. Map of pLKO1 containing an shRNA insert.


Tet Plko Puro载体 质粒图谱 优宝生物

99100 based on 16 PubMed citations.

. Tet-pLKO-puro Notes Feedback and protocol from Dr. We have made virus with the first of the vector constructs that we made our non-target shRNA control. In this study a single-vector lentiviral Tet-inducible shRNA system pLKO-Tet-On was generated to allow for the rapid generation of multiple stable cell lines with regulatable.

The original pLKO1-TRC cloning vector has a 19kb stuffer that is. ZERO BIAS - scores. Addgene inc plko tet puro vector Plko Tet Puro Vector supplied by Addgene inc used in various techniques.

Addgene inc tet plko puro vector Tet Plko Puro Vector supplied by Addgene inc used in various techniques. 99100 based on 29 PubMed citations. I agree there are differences in the cloning sites between the pLKO1 TRC and the Tet-pLKO1-puro.

First we modified the Tet-pLKO-Puro vector to make it easy EZ for molecular cloning EZ-Tet-pLKO-Puro. Our primary modification was to shrink the stuffer. 31 Map of pLKO1 puro.

There is that G missing at the end of the forward primer after the string of 5 Ts. The large stuffer 19 kb region between the cloning sites in the original Tet-pLKO-Puro vector necessitated the tedious process of gel purifying the vector fragment.


Addgene Tet Plko Puro Shkras


Plko 1 Puro 北京华越洋生物


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